Fibrinogen Immunohistochemistry – Mouse

Fibrinogen Immunohistochemistry – Mouse (PROTOCOL) Updated 04.24.19_ST FINAL

Citation

Davalos D, Ryu JK, Merlini M, Baeten KM, Le Moan N, Petersen MA, Deerinck TJ, Smirnoff DS, Bedard C, Hakozaki H, Gonias Murray S, Ling JB, Lassmann H, Degen JL, Ellisman MH, Akassoglou K. Fibrinogen-induced perivascular microglial clustering is required for the development of axonal damage in neuroinflammation. Nat Commun. 2012; 3:1227

[Pubmed citation]

Fibrinogen Immunohistochemistry – Human

Fibrinogen Immunochemistry – Human_MP (PROTOCOL) Updated 04.24.19_ST

Citation

Petersen MA, Ryu JK, Chang K-J, Etxeberria A, Bardehle S, Mendiola AS, Kamau-Devers W, Fancy SPJ, Thor A, Bushong EA, Baeza-Raja B, Syme C, Wu MD, Rios Coronado PE, Meyer-Franke A, Yahn S, Pous L, Lee JK, Schachtrup C, Lassmann H, Huang EJ, Han MH, Absinta M, Reich DS, Ellisman MH, Rowitch DH, Chan RJ, Akassoglou K. Fibrinogen activates BMP signaling in oligodendrocyte progenitor cells and inhibits remyelination after vascular damage. Neuron, 2017, 96:1003-1012

[Pubmed citation]

Fibrinogen Detection with Two-Photon Imaging

Fibrinogen Detection with Two-Photon Imaging

Fibrinogen Detection with Two-Photon Imaging (PROTOCOL) Updated 04.25.19_ST

Citation

Davalos D, Ryu JK, Merlini M, Baeten KM, Le Moan N, Petersen MA, Deerinck TJ, Smirnoff DS, Bedard C, Hakozaki H, Gonias Murray S, Ling JB, Lassmann H, Degen JL, Ellisman MH, Akassoglou K. Fibrinogen-induced perivascular microglial clustering is required for the development of axonal damage in neuroinflammation. Nat Commun. 2012; 3:1227

[PubMed citation]

Mouse iDisco Image

Fibrinogen – iDisco Mouse

Fibrinogen – iDisco Mouse (VR) (PROTOCOL) Updated 04.25.19_ST

Citation

Merlini M, Rafalski VA, Rios Coronado PE, Gill TM, Ellisman M. Muthukumar G, Subramanian KS, Ryu JK, Syme CA, Davalos D, Seeley WW, Mucke L, Nelson RB, Akassoglou K. Fibrinogen induces microglia-mediated spine elimination and cognitive impairment in an alzheimer’s disease model. Neuron 2019, 2019, 101:1099-1108

[Pubmed citation

Human-iDISCO

Fibrinogen – iDisco Human

Fibrinogen – iDisco Human (MM) (PROTOCOL) Updated 04.25.19_ST

Citation

Merlini M, Rafalski VA, Rios Coronado PE, Gill TM, Ellisman M. Muthukumar G, Subramanian KS, Ryu JK, Syme CA, Davalos D, Seeley WW, Mucke L, Nelson RB, Akassoglou K. Fibrinogen induces microglia-mediated spine elimination and cognitive impairment in an alzheimer’s disease model. Neuron 2019, 101:1099-1108

[Pubmed citation]

CD11b-Image

CD11b Fluorescent Immunostaining

CD11b fluorescent immunostaining protocol Updated 04.25.19_ST FINAL

Citation

Ryu JK, Rafalski VA, Meyer-Franke A, Adams RA, Poda SB, Rios Coronado PE, Pedersen LO, Menon V, Baeten KM, Sikorski SL, Bedard C, Hanspers K, Bardehle S, Mendiola AS, Davalos D, Machado MR, Chan JP, Plastira I, Petersen MA, Pfaff SJ, Ang KK, Hallenbeck KK, Syme C, Hakozaki H, Ellisman MH, Swanson RA, Zamvil SS, Arkin MR, Zorn SH, Pico AR, Mucke L, Freedman SB, Stavenhagen JB, Nelson RB, Akassoglou K. Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegeneration. Nat Immunol 2018, 19(11):1212-1223.

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In-Vivo-Imaging-of-Mouse

In Vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

In Vivo Imaging of the Mouse Spinal Cord Using Two-Photon Microscopy

Citation

Davalos D, Akassoglou K. In vivo imaging of the mouse spinal cord using two-photon microscopy. J Vis Exp. 2012. (59). pii: 2760

[Pubmed citation]  [Video]

 

Systemic Fibrinogen Depletion with Ancrod

Ancrod Pump Protocol Original Updated 06.12.19 ST1

Citation

Adams RA, Bauer J, Flick MJ, Sikorski SL, Nuriel T, Lassmann H, Degen JL, Akassoglou K. The fibrin-derived gamma 377-395 peptide inhibits microglia activation and suppresses relapsing paralysis in central nervous system autoimmune disease. J Exp Med, 2007, 204(3):571-82

[Pubmed citation]