Fibrinogen Immunohistochemistry – Human
Fibrinogen Immunochemistry – Human_MP (PROTOCOL) Updated 04.24.19_ST
Citation
Petersen MA, Ryu JK, Chang K-J, Etxeberria A, Bardehle S, Mendiola AS, Kamau-Devers W, Fancy SPJ, Thor A, Bushong EA, Baeza-Raja B, Syme C, Wu MD, Rios Coronado PE, Meyer-Franke A, Yahn S, Pous L, Lee JK, Schachtrup C, Lassmann H, Huang EJ, Han MH, Absinta M, Reich DS, Ellisman MH, Rowitch DH, Chan RJ, Akassoglou K. Fibrinogen activates BMP signaling in oligodendrocyte progenitor cells and inhibits remyelination after vascular damage. Neuron, 2017, 96:1003-1012.
Fibrinogen Detection with Two-Photon Imaging
Fibrinogen Detection with Two-Photon Imaging (PROTOCOL) Updated 04.25.19_ST
Citation
Davalos D, Ryu JK, Merlini M, Baeten KM, Le Moan N, Petersen MA, Deerinck TJ, Smirnoff DS, Bedard C, Hakozaki H, Gonias Murray S, Ling JB, Lassmann H, Degen JL, Ellisman MH, Akassoglou K. Fibrinogen-induced perivascular microglial clustering is required for the development of axonal damage in neuroinflammation. Nat Commun. 2012; 3:1227
Fibrinogen – iDisco Mouse
Fibrinogen – iDisco Mouse (VR) (PROTOCOL) Updated 04.25.19_ST
Citation
Merlini M, Rafalski VA, Rios Coronado PE, Gill TM, Ellisman M. Muthukumar G, Subramanian KS, Ryu JK, Syme CA, Davalos D, Seeley WW, Mucke L, Nelson RB, Akassoglou K. Fibrinogen induces microglia-mediated spine elimination and cognitive impairment in an alzheimer’s disease model. Neuron 2019, 2019, 101:1099-1108
Fibrinogen – iDisco Human
Fibrinogen – iDisco Human (MM) (PROTOCOL) Updated 04.25.19_ST
Citation
Merlini M, Rafalski VA, Rios Coronado PE, Gill TM, Ellisman M. Muthukumar G, Subramanian KS, Ryu JK, Syme CA, Davalos D, Seeley WW, Mucke L, Nelson RB, Akassoglou K. Fibrinogen induces microglia-mediated spine elimination and cognitive impairment in an alzheimer’s disease model. Neuron 2019, 101:1099-1108
CD11b Fluorescent Immunostaining
CD11b fluorescent immunostaining protocol Updated 04.25.19_ST FINAL
Citation
Ryu JK, Rafalski VA, Meyer-Franke A, Adams RA, Poda SB, Rios Coronado PE, Pedersen LO, Menon V, Baeten KM, Sikorski SL, Bedard C, Hanspers K, Bardehle S, Mendiola AS, Davalos D, Machado MR, Chan JP, Plastira I, Petersen MA, Pfaff SJ, Ang KK, Hallenbeck KK, Syme C, Hakozaki H, Ellisman MH, Swanson RA, Zamvil SS, Arkin MR, Zorn SH, Pico AR, Mucke L, Freedman SB, Stavenhagen JB, Nelson RB, Akassoglou K. Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegeneration. Nat Immunol 2018, 19(11):1212-1223.
In Vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
In Vivo Imaging of the Mouse Spinal Cord Using Two-Photon Microscopy
Citation
Davalos D, Akassoglou K. In vivo imaging of the mouse spinal cord using two-photon microscopy. J Vis Exp. 2012. (59). pii: 2760
Systemic Fibrinogen Depletion with Ancrod
Ancrod Pump Protocol Original Updated 06.12.19 ST1
Citation
Adams RA, Bauer J, Flick MJ, Sikorski SL, Nuriel T, Lassmann H, Degen JL, Akassoglou K. The fibrin-derived gamma 377-395 peptide inhibits microglia activation and suppresses relapsing paralysis in central nervous system autoimmune disease. J Exp Med, 2007, 204(3):571-82
